Hybridization
Hybrid Seed Production in Tomato

 Step #2. Pollen Collection

 collecting flowers
Collect flowers from the male parent to extract pollen. The best time for pollen collection is during the early morning before the pollen has been shed. Avoid pollen collection on rainy days.

 putting anther cones in envelopes
Remove the anther cones from the flowers and put them in glassine envelopes.

 drying anther cones
Dry the anther containers by placing them 30 cm below a 100-watt lamp for 24 hours. The lamp creates a drying temperature of about 30ºC. Pollen can also be sun-dried.

 prepare for shaking
Put the dried anther cones in a plastic pan or cup. Cover the cup with a fine mesh screen (200-300 mesh) and then seal it with a similar tight-fitting cup, serving as a lid.

 collecting pollen
Shake the cup about 10-20 times so that the pollen is collected in the “lid” cup.

 transferring pollen
Transfer the pollen into a small convenient-to-handle container for pollination. Fresh pollen is best for good fruit-set. It can be kept for one day at moderate room temperature.

When weather conditions are not suitable for pollination, dried or dehydrated pollen can be stored in a sealed container (capsule or vial) and kept in the freezer for about a month. Without freezing, the pollen can be kept in an ordinary refrigerator for 2-3 days without any significant loss in viability.

The pollen should be taken from the freezer and kept closed until the container warms to room temperature. This will prevent the pollen from getting wet due to condensation.
 

 


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